TCR Engineering and Validation Service
An integrated TCR service, integrating affinity maturation, in vitro functional
characterization, and in vivo evaluation, designed to support the development of TCR-T
cell therapies, particularly for solid tumors.
End-to-End TCR Discovery Platform
Integrated TCR Solution
Covering affinity maturation, in vitro functional evaluation and in vivo pharmacology avoiding fragmented development challenges.
Improved TCR Affinity
Effective affinity maturation platform to substantially improve affinity ( ~ 1800 fold).
Improved Function Predictability
Universal assay platform to allow a high-throughput for in vitro functional evaluation and specificity screening.
Reduced off Target Toxicity Risk
Superior assay sensitivity in detection of antigen independent T cell activation to mitigate off target toxicity risk.
TCR Discovery & Engineering Services for
TCR-T Cell Therapy
TCR Optimization
Functional Characterization
In Vivo Evaluation
TCR Optimization
TCR library construction, phage panning and screening:
- TCR design and expression validation in mammalian and prokaryotic platform
- Binding validation of TCR formats in phage system
- Binding validation of TCR formats in phage system
- Characterization of affinity matured TCR variants expressed in mammalian cells
50 TCR leads
Functional Characterization
Functional evaluation and specificity screening:
- Reporter cell based assay (RGA) based on TCR KO Jurkat
- Primary T cell based cytokine release
- Primary T cell based cytotoxicity
- Functional evaluation of TCRxCD3 bispecific by RGA
20 TCR leads
In Vivo Evaluation
In vivo efficacy validation:
- Animal model development and in vivo efficacy evaluation
- Pharmacokinetic for TCRxCD3 bispecific and in vivo persistence for TCR-T cells
- In vivo safety & early toxicity evaluation
2 TCR leads
Case Studies
Achieving 1,800-Fold Affinity Enhancement Through ProBio’s TCR Maturation Platform
Enhanced T-Cell Activation and Tumor Cell Killing Following TCR Affinity Maturation
Resources
BROCHURE
Biologics Discovery Services Brochure
Tailored discovery strategies for diverse biologics modalities, designed to enhance success rates.
FLYER
TCR Engineering and Characterization
Tailored discovery strategies for TCR engineering and characterization.
WHITE
PAPER
Discovery of Functional Antibody Leads
at Early Stage of Lead Screening
Solutions for antibody discovery platforms to overcome the limitations and improve efficiency of lead discovery.
FLYER
In Vivo CAR-T Discovery
Integrated service on in vivo car-t discovery: CAR leads/binder discovery, packaging, pharmacology study.
Frequently Asked Questions