Integrated ADC discovery strategy and antibody/protein cell line development expertise sharing

Biopharmaceuticals such as antibodies and recombinant proteins represent rapidly expanding categories of drugs offering new therapeutic applications. Achieving maximum production of these molecules is dependent on many factors such as suitable host cells, expression vectors and culture conditions.

Here, we describe ProBio’s cell line development platform (ProCLD) supporting biopharmaceutical manufacturing with high productivity and purity. An optimized vector pGenHT1.0-UP containing two “TiterUp” elements is utilized for boosting protein production. For bispecific/multispecific antibodies, optimized vector strategies have been used to modulate the relative expression of different polypeptide chains, thereby alleviating the chain mispairing issues. Notably, the turnaround time from transfection to PCB generation has been shortened to 8 weeks with highest titer of 10 g/L.

In summary, we show that ProBio has established superior CLD platform guaranteeing the accelerated and high-yielding cell line development.