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UproCHO Media Platform
UproCHO Cell Culture Media System
Optimize productivity and quality for high-performing production
The UproCHOMedia System is ProBio’s proprietary media system designed for ProBio’s cell line
CHOK1-GenS and proven to significantly optimize productivity and product quality in fed-batch, intensified
fed-batch and perfusion. All media components are manufactured in ISO 13485 certified facilities in
accordance with GMP practice. Combining UproCHOmedia withProBio’s CHOK1-GenS host cell and
high performing pGenHT 1.0-UP vector, we provide a platform ecosystem to enable our customers to
break through to next level of productivity.
High-expression
UproCHO
media system
High-throughput &
automatic screening
platform
vectors:
CHOK1-GenS
CHOK1-ADCC+
pGenHT 1.0-UP
pGenHT 1.0-DGV
ProBio’s ecosystem of CHO expression
UproCHOmedia system highlights
Developed for CHOK1-GenS
High Productivity
Cost Efficiency
Proven to optimize cell growth,
productivity and product quality
when used in CHOK1-GenS
Titer improvement in>90% projects
Average titer improvement 49%,
max. 117%
Competitive COGS
Additional incentives for the case
where GMP manufacturing is in
ProBio
CHOK1-GenS cells cultured in UproCHO media demonstrated
improved protein production
Titer performance of UproCHOmedia
mAb
Bispecific Ab/Proteins
15000
10000
5000
0
A
B
C
D
E
F
G
H
I
J
UproCHO
Brand A
Brand B
ProBioCDMO.com
+1-732-885-9188
Accelerating Ideas. Delivering Impact.
Case study of UproCHOmedia
In the bsAb CMC project, the titer reached 9g/L by using UproCHOfed-batch media system and
the key quality attributes showed stable in the process scale up from 3L to 200L.
Time (day)
Time (day)
SEC(%)*
CE-SDS NR(%)*
Main peak
94.16
icIEF(%)*
Glycan(%)*
Man5
Scale
Titer(g/L)
Main peak
Acidic peak
48.8
Main peak
40.1
Basic peak
11.1
1.23
200L
3L
9.17
94.48
92.98
9.60
38.5
10.6
1.29
93.93
50.9
* The analysis is based on 1-step purification samples.
UproCHOcell culture media and feed products
Media system
Media
Product name
UproCHOM01
UproCHOF01A
UproCHOF01B
UproCHOM02
Physical state
Package size
Powder
Powder
Powder
Powder
5L~100L
1L~20L
1L~10L
Fed-batch basal media
Fed-batch feed A
Fed-batch feed B
Perfusion basal media
Fed-batch/
intensified
fed-batch
media
Quote
Perfusion
media
UproCHOP01A
UproCHOP01B
Perfusion mix media
Powder
Quote