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ProSyn™ Linearized DNA Using Cell-free Technology
TM
ProSyn Linearized DNA
Using Cell-free Technology
ProSynTM Linearized DNA
Cell-free technology is a breakthrough in plasmid and linearized DNA manufacturing with its short
turnaround time. ProSynTM Linearized DNA is using enzymatic DNA synthesis through rolling circle
amplification (RCA). Under the action of proprietary PowerStarryTM Primer Pool, ProBio is able to accelerate
different projects by reducing time and manpower.
Template Plasmid
Rolling Circle Amplification (RCA)
Single strand for
template plasmid
Phi29 DNA polymerase
PowerStarryTM primer
pool
Monomer size
ProSynTM
Linearized DNA
Amplified DNA
Endonuclease
Endonuclease Digestion
No IP issue
Non cell bank
Unparalleled Accuracy: low error rate
PowerStarryTM Primer Pool: high universality with stable structure
Deliver small quantity of GMP product in fast turnaround time
Comparison of E.coli Fermentation and Cell-free Technology
Linearized DNA
ProSynTM Linearized DNA
E. coli Fermentation
Technology
Cell-free technology
(with linearization)
Plasmid Length
Cell Bank
Up to 15kb
Yes
Up to 15kb
No
Minimum Mfg. Scale
Yield
10L (g level)
Up to 1g/L
10~12 weeks
10mL (1mg)
2~3 mg per 10 ml
1~2 weeks
TAT
ProBioCDMO.com
+1-732-885-9188
Accelerating Ideas. Delivering Impact.
ProSynTM Linearized DNA Performance
2~3 mg per 10 ml with 4000~5000x
Purity (IEC-HPLC): ~95%
amplification rate
300
250
200
150
100
50
0
10mL
50mL
RCA Scale
100mL
E. coli fermentation vs cell-free technology
14
12
10
8
120.00%
100.00%
80.00%
60.00%
40.00%
20.00%
0.00%
6
4
2
0
E.coli
Cell-free
E.coli
Cell-free
IVT-mRNA yield
IVT-mRNA capping efficiency
ProBio ProSynTM Linearized DNA Service Overview
IND
C
Phase
I
Phase
II
Phase
III
IIT
IND
BLA
Commercial
GMP Grade
ProSynTM
Linearized DNA-
PreCMC Grade
GMProTM Grade
GMP Grade
BLA PC/PV
IND CMC