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ProSpeed™ Single B Cell Antibody Discovery Service
TM
ProSpeed Single B Cell Antibody
Discovery Service
Fast and cost-effective discovery of functional antibody leads in 1 month!
Integration of the ProSpeed expression with Beacon® platform
Get Functio1naml Anotibnotdhy sSe!quence in
Immunization
B cell harvest and enrichment
•
Multiple, sequential in-chip
screening
Import/penning single B cell
(tens of thousands of B cells)
completed in 1 day
•
2-3 weeks(ProSpeed™ expression) vs
4-6 weeks (plasmid-based expression)
(from B cell exporting to final validated
Ab sequences acquisition)
EXPEDITED
TIMELINE
In-chip assays:
Deep screen for specificity, functionality
Forward functional screening for the
largest B cell pool possible → Minimal
diversity loss
•
•
Export single B cell
High-throughput binder assays using
ProSpeed™ expression, avoid missing
high-quality leads!
HIGH
DIVERSITY
ProSpeed™ expression
Binding/ functional confirmation assays
• Delivery of over 150 projects
• Extensive experience in various
targets
Sequencing and Ab production
EXTENSIVE
EXPERIENCE
Available for Discovery of mAb and sdAb
A Perfect Combination of ProSpeed™ expression and Beacon® Platform
•
•
Higher throughput Ab expression and assay
®
Beacon
Breaking -through the technical limitations of Beacon platform with:
Compatibility with various functional assays
Platform
Feasibility for multiple rounds of screening beyond Beacon’s limits
Improved assay sensitivity
Potential “rescuing” of missed hits due to unsuccessful RT
ProSpeedTM
Expression
-PCR
•
Reduces by more than 50%, the cost and time of obtaining
functional antibodies
ProBioCDMO.com
+1-732-885-9188
Accelerating Ideas. Delivering Impact.
Case Study: DLL3 mAb Discovery by ProSpeed™ Ab Discovery
Linear cassette
Plasma B
cell loading
3 Antigen protein
binding
Cell-based
binding
Recombinant
mAb validation
Sanger/NGS
Sequencing
based
expression
20+ binders
100+ binders
50+ binders
40+ binders
Up to 4 sequential assays were
Phylogenetic tree
included in the screening workflow
Sequences discovered from
Single B screening (green)
are highly diverse and
clustered to many different
branches of the
•
Assay Type
Human DLL3
Positive Binders
98
127
58
50
Cyno DLL3
Mouse DLL3
Human DLL3 cell
phylogenetic tree.
Exported Cross
Unique Sequence
61 out of 100
21 out of 23
Various binding affinity to cell surface DLL3 and different binding epitopes
Case Study: sdAb Discovery by ProSpeed™ Ab Discovery
1 Day
2-3 Months
2 Weeks
1-2 Days
ProSpeed
expression/
validation
Alpaca
immunization
PBMC
isolation
Single B cell
screening
Single B cell
sequencing
Ag specific B cell enrichment
3-4 rounds in -chip
screening assays
Linear expression and sdAb sequence
recovered from single B cell
Protein/DNA/cell line
Screening result
Screening
PCR Successful
Positive
Immunization
Exported Clones
Sequenced clones
Unique Seq.
Seq.
51
VHH
84
IgG
37
3rd
149
153
68
92
57
84
4th
95
21
75
Phylogenic tree analysis of CDR AA sequence of hu/cyno Ag binders
Good sequence diversity:
• Only one identical unique
sequence found from
screening the B cells of the 3rd
and 4th immunization.
•
Unique sequences identified
from 3rd and 4th immunizations
evenly distributed to
overlapping clusters.