Poster
CHO Media Platform for Titer, Viability, and Consistency
UproCHO™ media platform:
Taking your molecular titer and quality to the next level
5 Core Benefits of UproCHO™
Introduction
UproCHO
Benefit 1 Guaranteed Low Cost
· UproCHO is customized for ProBio’s host cells
Saving up to 70% on medium costs.
•
and vectors.
pGenHT-UP / DGV
· UproCHO is characterized by animal origin-free,
protein-free and chemically defined.
Benefit 2 Superior Titer Performance
ECACC CHOK1-
Gens / ADCC+
· UproCHO media platform includes several
dry powder products and a titer/quality
adjusting toolbox.
Pushing the titer limits of mAb and bispecific Ab under
Fed-batch mode to 14g/L and 10g/L.
•
Performing the highest titer in 19 of 26 (approx. 73%) projects
among all tested medium brands.
•
UproCHO Series Dry Powder
Fed-batch/
Intensified Fed-batch
Perfusion
™
Brand D
Brand B
Brand C
Brand A
Titer comparison on 26 projects
UproCHO Process
CMA: Vitamins
Amino Acids
Benefit 3 Minimize the Risk of PK Issues
Trace Elements
Inorganic Salts
High mannose (Man5 and Man8/9) showed approximately
3-fold faster drug clearance than complex-fucosylated
glycoforms, i.e., higher Man5, shorter drug half-life.
UproCHO exhibited lower Man5 proportion than other
media competitors.
•
Media Toolbox
UproCHO Process
Based on UproCHO
•
Man 5 comparison on multiple projects
UproCHO
BrandA
BrandB
BrandC
UproCHO Media Toolbox Showcases
Case 1: Increasing the titer of one BsAb molecule
by optimizing the amino acids concentrations
Man 5 comparison of
UproCHO and other brands
Pharmacokinetic profiles of mAb variants
in Harlan athymic nude mice
By rebalancing the concentrations of amino acids
of the medium, the titer can be increased by 26%
compared with the control group.
Benefit 4 Consistent Batch-to-batch Stability
and Stable Supply Chain
Dual-supplier mode ensures stable supply.
•
•
The prior OEM vendor is Thermo Fisher UK facility.
Benefit 5 Provide Customized Media
Development Services
Metabolites with significant correlation with qP were enriched in
Amino acids enter TCA through
different pathways
TCA cycle and Ala, Asp, Gln metabolism
• Media toolbox based on UproCHO supports process
development, process change and biosimilar development.
Case 2: Reducing the acidic species by lowering the content of cysteine
By reducing the cysteine content, the acidic species proportion of the 5 tested
projects decreased to varying degrees.
References
1. A Metabolomics Approach to Increasing Chinese Hamster Ovary (CHO)
Cell Productivity. Metabolites.2021, 11, 823.
2. Impact of CHO Metabolism on Cell Growth and Protein Production: An
Overview of Toxic and Inhibiting Metabolites and Nutrients. Biotechnol
J. 2018, 13(3), e1700499.
3. Cysteine in cell culture media induces acidic IgG1 species by disrupting
the disulfide bond network. Biotechnology and Bioengineering. 2021,
118, 1091–1104.
In vitro cysteine treatment induces acidic variants by
breaking disulfide bonds and introducing cysteinylations
4. Production, characterization and pharmacokinetic properties of antibodies
with N-linked Mannose-5 glycans. MAbs.2021, 4(4), 475-87.