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ADC Discovery Service
ADC Discovery Service
One-stop solution from target to preclinical candidates (PCC)
High-throughput internalization assay screening with antibodies supernatant
Antibody-Drug Conjugates (ADCs) are a class of targeted cancer therapies that combine the specificity of
monoclonal antibodies with the potency of cytotoxic drugs. ADCs work by binding to specific antigens on cancer
cells and delivering the attached cytotoxic drug directly into the cancer cell, resulting in selective killing of cancer
cells while sparing healthy cells. Over the years, significant research efforts have been devoted to improving the
efficacy and safety of ADCs, leading to a range of novel ADC designs and optimization strategies.
With 19 years of experience in antibody drug discovery, ProBio offers one-stop ADC discovery services to
accelerate customers’ R&D speed from target to preclinical candidate(PCC), and seize the hottest development
track.
Balancing Functionality And Safety
One-stop ADC Discovery Solution
Excellent Track Record
High-throughput internalization assay
screening with antibodies supernatant
Conduct pharmacology studies before
antibody humanization to evaluate
efficacy and safety
Diverse antibody discovery technical capaigns
100+ ready-to-use payload-linker conjugates
9 types of ADC bioassay methods
One-stop in vivo pharmacology solution for
ADC
100+ ADC naked antibody discovery
project experience
100+ ADC bioassay project experience
From Targets To Preclinical Candidate Molecules
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Target Evaluation
With 20+ discovery
project experiences in
ADC targets, including
HER2, EGFR, and TROP2
Payload-linker Library
100 cytotoxins
700+ linkers
100+ ready-to-use payload-
linker conjugates
In Vitro Bioassay
Developability
9 types of ADC bioassay methods：
√ Live-cell imaging-based Internalization;
Tm,Tagg, purity, solubility,
thermal stability, freeze-thaw
stability, etc.
√ Toxin-conjugated mAb based cytotoxicity assay;
√ Bystander effect study
100+ project experience in antibody
internalization bioassay
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Naked Antibody Discovery
ADC Conjugation
In Vivo Pharmacology
One-stop Service:
Pharmacodynamics, Pharmacokinetics,
Toxicology
200+ tumor cell banks
80+ tumor cell models
Covering 24 tumor indications
Diverse antibody discovery technical campaigns:
Hybridoma, phage display, single B cell screening, fully human
antibody discovery
Lysine-based
Cysteine-based
Site-specific glycan conjugation
Purity by SEC-HPLC
Different biologics modalities mAb，bsAb，sdAb, antibody fragment
Functional assay in the screening stage:
√ High-throughput internalization assay screening with
antibodies supernatant (>100 Abs)
√ Epitope binning to preserve the diversity of binding epitope
DAR by HIC-HPLC
Naked Antibody Discovery Platform
Animal
With 20 years of antibody discovery experience, GenScript ProBio offers
customers with a comprehensive route to naked antibody discovery,
improving project success.
Immunization
Starting
Material
ProBio conducts high-throughput internalization screening with antibodies
supernatant to identify antibody leads with desirable internalization
capability in the early discovery stage.
Premade Phage
Display libraries
Single B Cell
Screening
Hybridoma
Phage Display
Technical
Platforms
ProBioCDMO.com
+1-732-885-9188
Accelerating Ideas. Delivering Impact.
In Vitro Bioassay Platform for ADC
ProBio offers 9 diversified ADC detection methods to provide customers with diverse, efficient, and stable ADC bioassay
platforms. In addition to traditional internalization detection based on pH probes and cell surface secondary antibody tracking,
ProBio has upgraded ADC internalization detection schemes, providing toxin-conjugated mAb based cytotoxicity assay applica-
ble for hybridoma supernatant screening and live-cell imaging based internalization. ProBio can also provide customers with
bystander effect experiments to evaluate ADC efficacy and potential risks.
Cytotoxicity
Scope
Type
Internalization assay
Bystander effect
assay
Toxin-conjugated mAb based
cytotoxicity assay
Live-cell
pH-Indicator Temperature
Cell growth
inhibition assay
Co
-
culture assay
Medium
transfer assay
imaging based based
shift based
Internalization Internalization Internalization
(pHrodo)
VHH-DT
DT3C
/MMAE
ADC or Toxin
Microplate
Reader / Live- Flow cytometer
Cell Analyzer
Molecule
Analyzer
Naked antibody
Live-Cell
Analyzer
(IncuCyte)
ADC
Live-Cell
Analyzer
Microplate
Reader
Microplate
Reader
Microplate
Reader
FACS
FACS
High
High sensitivity
Cost-effective
Susceptible to
multiple factors
throughput
High throughput
Feature
Real-time and Specific
kinetics analysis
Cytotoxic MOA Cost-effective
Real-time
Specific
Cost-effective
Close to the Close to the
cytotoxic MOA cytotoxic MOA
Specific
Purified
antibody
or LEC
Purified
Test
sample
antibody
Purified
antibody
Purified
antibody
Purified
antibody
Purified
antibody
Purified
antibody
Purified
antibody
Purified
antibody
or Hybridoma
supernatant supernatant
80+ positive reference antibody dose-response curve (freely available)
Target cells 200+ tumor cell lines and 100+ overexpression cell lines were provided free of charge
In Vivo Pharmacology for ADC
ProBio offers a range of in vivo pharmacology service, including animal-based in vivo efficacy, pharmacokinetics,
toxicology research, and biomarker-based detection, to meet the compliance requirements for new drug applica-
tions, and help you achieve faster, better, and more cost-effective new drug development.
One-stop ADC in vivo pharmacology solution
Key Material
Screening
Ex & In Vivo
PK Package
Ex & In Vivo
Toxicity
ADC Preparation
In Vivo Efficacy
Extensive model resource
Pharmacokinetic experiments
Toxicological studies
Abundance of mouse strains
6 tumor bearing animal models
80+ Tumor cell models
200+ Tumor cell banks
24 Tumor indications
Method development and validation
Pharmacokinetic experiments in
cynomolgus monkeys and other primates
Tissue distribution experiment
Toxicity with a single administration
Dose exploration experiments
Long-term toxicity testing
Local toxicity
Receptor mass experiments
Tissue cross-reactivity experiments