1. What are the differences between various antibody discovery platforms?

Different antibody discovery platforms vary mainly in how B cells are sourced, how antibodies are screened. Hybridoma technology uses immunized animals and fuses B cells with myeloma cells to generate stable antibody-producing clones. It is reliable and well-established but relatively time-consuming and lower throughput. Single B cell screening platforms directly isolate antigen-specific B cells and obtain paired heavy and light chain sequences. This approach is faster, higher throughput, and preserves natural antibody pairing, enabling rapid discovery of functional antibodies. Display technologies (e.g., Phage Display, yeast or mammalian display) use large in vitro libraries to screen antibodies against targets. They provide very large diversity and do not require immunization, but may require additional steps to optimize affinity and functionality.

2. How should we select the most appropriate antibody discovery platform?

Selection depends on project goals, timeline, and target characteristics. Factors to consider include: Diversity Needed (Moderate to High), Timeline (Slower to Fast), Natural Pairing (Yes or No), Species (limited to Any species), and Throughput (Low to High). Hybridoma offers natural pairing but is slower with low throughput. Single B Cell offers a fast timeline, high throughput, and natural pairing. Display technologies provide high diversity and can use any species, but lack natural pairing.

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Diverse SdAb Discovery Approaches

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SdAb naïve and immunized library

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Poster: Utilization of Naive VHH Phage Display Library & Integrated Antibody Engineering Platform to Generate Antibody Leads for CAR-T Therapy

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Molecule Focus SdAb (VHH)

SdAb Discovery

Poster: The Discovery of a CD3 Agonistic SdAb to Accelerate the Development of CD3 T cell Engager Multi-specific Antibodies

White Paper: Well-suited Antibody Discovery Strategy for CAR T Cell Therapy

Diverse SdAb Discovery Approaches

Poster: Utilization of Prospeed™ Expression System to Empower Single B Cell Based Antibody Discovery Platform

Flyer: Prospeed™ Single B Cell Antibody Discovery Service

On-demand Webinar: Integrated SdAb Discovery via Phage Library and Single B Cell Screening Technology

SdAb naïve and immunized library

Poster: Utilization of Naive VHH Phage Display Library & Integrated Antibody Engineering Platform to Generate Antibody Leads for CAR-T Therapy

On-demand Webinar: Integrated SdAb Discovery via Phage Library and Single B Cell Screening Technology

On-demand Webinar:

Integrated SdAb Discovery via Phage Library and Single B Cell Screening Technology

On-demand Webinar:

Integrated SdAb Discovery via Phage Library and Single B Cell Screening Technology